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Expressed sequence tags for molecular systematics

Reference	G14548
Principal Investigator / Supervisor	Professor Alfried Vogler
Co-Investigators / Co-Supervisors	Dr Michael Caterino, Dr Peter Foster
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	260,696
Status	Completed
Type	Research Grant
Start date	01/03/2001
End date	01/03/2004
Duration	36 months

Abstract

Molecular systematics have been limited by the difficulties of obtaining multiple loci from taxonomically diverse organisms. As an alternative to current degenerate primer techniques, we explore the possibility of producing comparative sequence information by cloning large numbers of expressed sequences and using high-throughput DNA sequencing. We test this approach across different hierarchical levels in the diverse order Coleoptera (beetles). The work will extend the number of genes available to molecular systematics and will realistically include a largely complete set of sequences for approx. 50 loci for key taxa of beetles and will permit addressing important questions in molecular evolution and systematics. The approach can be applied to any group or organisms and provides a scaffold for extended analysis of comparative genomics.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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