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To determine the effect of securin separin and cohesin dysfunction on genome stability and apoptosis in human cells

ReferenceG14108
Principal Investigator / Supervisor Professor Stephen Taylor
Co-Investigators /
Co-Supervisors
Institution The University of Manchester
DepartmentLife Sciences
Funding typeResearch
Value (£) 187,836
StatusCompleted
TypeResearch Grant
Start date 01/04/2001
End date 01/07/2004
Duration39 months

Abstract

The establishment, maintenance and ultimately, the timely loss of sister chromatid cohesion is essential for genome stability as it ensures accurate chromosome segregation. In yeast, loss of cohesion is accomplished by Esp1/Cut 1 separin mediated cleavage of the Scc1 cohesion. To prevent premature loss of cohesion prior to chromosome alignment, separins are inhibited by Pds1/Cut2 securins. Cohesin, separin and securin related proteins have recently been identified in human cells but their functional roles have yet to be addressed. Therefore, we will manipulate the cohesin/separin/securin mechanism in human cells by expression of appropriate mutants and determine the effects on sister chromatid cohesion, the exit from mitosis and apoptosis.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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