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Probing the molecular events involved in directed DNA melting by sigma54 and its B. subtilis homologue sigma L
Reference
G14075
Principal Investigator / Supervisor
Professor Martin Buck
Co-Investigators /
Co-Supervisors
Professor David Smith
,
Professor Peter Stockley
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
164,140
Status
Completed
Type
Research Grant
Start date
01/01/2001
End date
30/06/2004
Duration
42 months
Abstract
We will use fluorescence spectroscopy, surface plasmon resonance and ultracentrifugation to investigate the molecular details of promoter DNA melting by the activator-dependent sigma factors, sig54 and sigL. Fluorophores will be incorporated into both the DNA, via 2-aminopurine substitution, and the protein, via covalent modification of site-specifically inserted cysteine residues. These experiments will be used to derive the kinetic rates for the various steps involved in sigma induced isomerisation, as well as probing the concomitant conformational changes, e.g. via FRET.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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