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Global analysis of gene regulation at the levels of transcription initiation and messenger RNA decay

Reference	G14070
Principal Investigator / Supervisor	Professor Peter Stockley
Co-Investigators / Co-Supervisors	Dr Kenneth McDowall
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	198,276
Status	Completed
Type	Research Grant
Start date	01/02/2001
End date	01/02/2004
Duration	36 months

Abstract

We will use the latest gene array technology (GAT) to determine to what extent three transcriptional regulators of amino acid metabolism, i.e. MetJ, ArgR and TrpRm impact E. coli gene expression on a genome-wide basis. This will provide a platform to assess for the first time the global effects of mutations that through structural and functional studies are known to have altered co- operativity, sequence recognition, multimerisation or co-repressor binding. Additionally, we will use GAT to determine a stability profile for the E. coli transcriptome, which in turn will provide an unprecedented opportunity to examine the integrated regulation of transcription and mRNA decay and determine the influence of individual RNA-decay factors on the stability of each member of the E. coli mRNA pool.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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