Award details

X-ray crystallographic analysis of amidase from Pseudomonas aeruginosa

Reference	G13570
Principal Investigator / Supervisor	Dr Paul Brown
Co-Investigators / Co-Supervisors	Professor Brian Sutton
Institution	King's College London
Department	GKT School of Biomedical Sciences
Funding type	Research
Value (£)	10,674
Status	Completed
Type	Research Grant
Start date	01/07/2000
End date	01/01/2002
Duration	18 months

Abstract

Cryo-methods will be used to stabilise crystals of Pseudomonas aeruginosa amidase and isomorphous replacement derivatives or a selenomethionine version of amidase will be used to begin the determination of the 3D structure of the enzyme by X-ray crystallography. Improvements to crystal growth will be sought by changes in amidase purification methods and by substitution of a reactive cysteine residue by site-directed mutagenesis. Crystals of evolved amidases with novel substrate specificities, of amidases with altered substrate binding and of amidase complexed with the active-site directed inhibitor, urea, will be obtained.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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