Award details

Turnover profiling: commitment and degradation of the yeast proteome

Reference	G13495
Principal Investigator / Supervisor	Professor Robert Beynon
Co-Investigators / Co-Supervisors	Professor Simon James Gaskell, Professor Stephen Oliver
Institution	University of Liverpool
Department	Veterinary Preclinical Science
Funding type	Research
Value (£)	400,944
Status	Completed
Type	Research Grant
Start date	03/01/2001
End date	03/12/2004
Duration	47 months

Abstract

Protein degradation is a key process in the control of protein concentration inside the cell, and in eukaryotes is largely initiated by ubiquitin conjugation. However, the targeting of ubiquitin, and attendant targeting motifs, are largely unknown. In this study, we propose novel proteome-based strategies to determine, in yeast, the specific lysine residue that is conjugated by ubiquitin, and for turnover profiling; to measure the rates of turnover of many hundreds of proteins from S. cerevisiae simultaneously. The work offers a new perspective on the selectivity and delivery of intracellular protein degradation, and yields new information on proteome dynamics. We will monitor proteome turnover and labelling under different growth conditions, notably nitrogen and carbon limitation and anaerobiosis, for which transcriptome and steady-state proteome data will be available.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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