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Mechanisms of transcriptional regulation by Escherichia coli FNR protein
Reference
G13155
Principal Investigator / Supervisor
Professor Steve Busby
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
198,276
Status
Completed
Type
Research Grant
Start date
01/06/2000
End date
01/06/2003
Duration
36 months
Abstract
The overall aim is to understand how FNR-dependent promoters that carry a DNA site for FNR near position -41 are organised, to understand how these promoters differ from CRP-dependent promoters with a similar architecture, and to understand the mechanisms by which FNR-dependent transcription activation is down-regulated by upstream-binding factors. Working with an FNR- dependent promoter carrying a DNA site for FNR near position -41, we propose to: 1) identify the determinants of alpha CTD that make contact with FNR during transcription activation; 2) determine the location of the two alpha CTDs and the organisation of transcriptionally-competent complexes; 3) investigate the consequences of binding a second FNR molecule upstream; 4) determine the mechanism by which upstream-bound FNR inhibits FNR-dependent transcription activation; and 5) investigate the consequences of upstream binding of CRP or FIS.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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