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Transcription activation by CRP at simple and complex promoters
Reference
G13154
Principal Investigator / Supervisor
Professor Steve Busby
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
214,584
Status
Completed
Type
Research Grant
Start date
01/06/2000
End date
31/08/2005
Duration
63 months
Abstract
The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at a large number of simple promoters by making a direct contact with the C-terminal domain of the alpha subunit (alpha CTD), of RNA polymerase holoenzyme (RNAP). In previous studies we have identified the surfaces of both CRP and alpha CTD responsible for this contact. Other studies have shown that, although CRP is a dimer and although RNAP contains two alpha CTDs, at the lac promoter, full transcription activation requires interaction between only one CRP subunit and one alpha CTD. The objectives of the proposed study are: 1) to use suppression genetics to investigate the CRP- alpha CTD interface; 2) to show that, in contrast to the situation at the lac promoter, both alpha CTDs are essential for CRP-dependent activation at some simple CRP-dependent promoters; and 3) to investigate complex promoters, based on lac promoter architecture, where a second upstream CRP molecule promotes transcription activation by interacting with the spare alpha CTD.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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