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Determining the role of Spo11p and DSB formation in signalling the onset of meiotic chromosome pairing
Reference
G12839
Principal Investigator / Supervisor
Professor Alastair Goldman
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
193,936
Status
Completed
Type
Research Grant
Start date
30/05/2000
End date
30/05/2003
Duration
36 months
Abstract
During meiosis, in most organisms chromosome pairing and recombination are mechanistically linked, but the nature of their independence is not well understood. In budding yeast, the formation of meiotic DNA double strand breaks (DSBs) is a pre-requisite to both recombination and chromosome pairing. The aim of the study is to determine whether it is DSBs themselves which provide the signal to the cell to commence chromosome pairing or if the DSB forming enzyme Spo11p is required to initiate the signal. This will be done using Spo11p independent DSBs created by site specific endonucleases. We will also set up screen for spo11 mutants which can make DSBs but cannot induce chromosome pairing. Chromosome pairing will be assessed both genetically and cytologically.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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