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Functional analysis of the Arabidopsis microtubule associated protein map-65 in vivo

Reference	G12762
Principal Investigator / Supervisor	Professor P Hussey
Co-Investigators / Co-Supervisors	Professor Clive Lloyd, Dr Andrei Smertenko
Institution	Durham University
Department	Biological and Biomedical Sciences
Funding type	Research
Value (£)	200,676
Status	Completed
Type	Research Grant
Start date	01/03/2000
End date	11/06/2004
Duration	51 months

Abstract

We have cloned a cDNA encoding a member of the tobacco MAP-65 family of microtubule associated proteins. The sequence shows no significant identity with other known MAPs and therefore represents a novel MAP sequence. Comparison on Arabidopsis databases identified three divergent MAP-65 genes (41-44 percent amino acid identity with each other) and we have cloned and sequenced the corresponding cDNAs. To address the function of these proteins in vivo, we will assess the phenotypes of transgenic Arabidopsis expressing antisense and sense MAP-65 genes under the control of the Alc switch. In addition we will microinject the recombinant proteins into plant cells. The intracellular and intercellular distribution of the three MAP-65 proteins will be assessed by immunofluorescence microscopy using isotype specific antibodies and MAP-65/GFP chimeras in transgenic Arabidopsis.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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