BBSRC Portfolio Analyser
Award details
Isolation and functional characterisation of novel T-box genes in the zebrafish (D. rerio)
Reference
G12140
Principal Investigator / Supervisor
Professor Philip Ingham
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Biomedical Science
Funding type
Research
Value (£)
272,557
Status
Completed
Type
Research Grant
Start date
01/08/1999
End date
14/02/2003
Duration
42 months
Abstract
We have recently isolated a number of novel zebrafish T-box (tbx) containing sequences. Many of these are expressed in patterns suggestive of a role in the development of mesodermal derivatives, including notochord, somites and fin bud mesenchyme. Here we propose to characterise their expression and function during early development of wild type and mutant zebrafish embryos. The function of each gene will be analysed using RNA injections of the tbx-specific interfering double stranded RNAs (RNAi) or RNAs encoding dominant negative protein forms. To facilitate the identification of candidate mutations, each gene will be mapped using radiation hybrid panels. In the case of thx5, we will specifically look for linkage to a newly isolated mutation neckless. Finally, the regulation of these tbx genes will analysed using mutations that affect mesoderm development.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search