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The role of nucleosome assembly protein 1 like (NAP1L) in haematopoiesis
Reference
G12014
Principal Investigator / Supervisor
Professor Matt Guille
Co-Investigators /
Co-Supervisors
Professor Roger Patient
,
Dr Alan Thorne
Institution
University of Portsmouth
Department
Sch of Biological Sciences
Funding type
Research
Value (£)
162,192
Status
Completed
Type
Research Grant
Start date
04/01/2000
End date
03/01/2003
Duration
36 months
Abstract
NAP1L RNA is expressed in the ectoderm overlying the ventral blood islands and its overexpression increases globin mRNA levels. We will study the role of NAP1L in early blood development first by analysing its distribution by immunohistochemistry, then we will use overexpression to test whether globin control by xNAP1L is cell autonomous. To examine the mechanism by which xNAP1L increases globin RNA we will overexpress it and test the levels of molecules known to be important for blood formation. If necessary we will use overexpression and transgenesis to identify globin sequences needed for the regulation. Finally we will test whether nucleosome assembly is necessary for globin control by assaying mutated xNAP1L for both these activities.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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