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Mechanism of alternative splicing at an atypical splice donor in the vertebrate fibroblast growth factor receptor genes

Reference	G11717
Principal Investigator / Supervisor	Professor Gavin Screaton
Co-Investigators / Co-Supervisors	Professor Alex Wilkie
Institution	University of Oxford
Department	Clinical Medicine
Funding type	Research
Value (£)	165,480
Status	Completed
Type	Research Grant
Start date	01/07/1999
End date	01/07/2002
Duration	36 months

Abstract

We intend to study a novel, conserved 5' splice site (ACA/GAAAGT) involved in alternative splicing of Fibroblast Growth Factor Receptor 1, 2 ad 3 pre-mRNAs. The FGFR protein translated from mRNA that has used this splice site contains a Valine-Threonine dipeptide that is absent when splicing proceeds from a competing upstream conventional GT 5' splice site. This VT dipeptide is a target for protein kinase C phosphorylation, and functional differences between the VT+ and VT- forms assayed in vitro suggests that this alternative splicing event is important for the regulation of FGFR function. We propose to investigate the mechanisms of splicing at this GA splice site by: i) documenting the pattern of alternative splicing in a variety of different cells, ii) mutagenesis of the conserved sequence, and iii) biochemical characterisation of the factors required.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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