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Mechanisms of gene delivery to angiogenic tissues
Reference
G11224
Principal Investigator / Supervisor
Dr David Berk
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Manchester Pharmacy School
Funding type
Research
Value (£)
178,093
Status
Completed
Type
Research Grant
Start date
01/02/1999
End date
01/10/2001
Duration
32 months
Abstract
To understand mechanisms for the disposition of systemically administered genes, several novel imaging techniques will be used for the continuous noninvasive monitoring of gene delivery and expression kinetics in intact tissue at the microscopic level. The techniques will be used to observe the uptake of lipid/DNA complexes into the endothelial cells and parenchymal cells of normal mouse skin and in tumour and wound tissue undergoing angiogenesis. Fluorescence Recovery After Photoactivation (FRAP), Fluoresence Correlation Spectroscopy, and image deconvolution will be used to assess the penetrability of the extravascular space. Expression of GFP protein will be monitored to directly compare gene delivery and the kinetics of gene expression.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
BioImaging (BI) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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