BBSRC Portfolio Analyser
Award details
The molecular mechanism for the induction of nucleosome phasing
Reference
G10898
Principal Investigator / Supervisor
Professor Constanze Bonifer
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
School of Medicine
Funding type
Research
Value (£)
144,768
Status
Completed
Type
Research Grant
Start date
01/05/1999
End date
01/05/2002
Duration
36 months
Abstract
We have been studying the chicken lysozyme locus as a model to examine the prerequisites for the establishment of a transcription competent chromatin structure. We recently discovered a new enhancer element located at 3.9 kb which is activated early in macrophage differentiation. The chromatin of the 3.9 kb enhancer exhibits an unusual structure, since its activation leads to nucleosome phasing over 3 prime flanking sequences. In vivo DNA-protein interaction studies demonstrated differential binding of transcription factors. In this proposal we wish to answer the question, whether specific factor(s) and/or a specific DNA-structure is responsible for the precise positioning of nucleosomes after enhancer activation. We wish to examine the molecular basis for the tissue specific activity of this interesting cis-regulatory element.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search