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Natural variation among restriction enzymes
Reference
G10881
Principal Investigator / Supervisor
Professor Steve Halford
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Biochemistry
Funding type
Research
Value (£)
213,556
Status
Completed
Type
Research Grant
Start date
01/02/1999
End date
01/02/2002
Duration
36 months
Abstract
Most type II restriction enzymes are dimers that cut duplex DNA at palindromic sites. Each active site in the protein is placed to cut one strand, so one turnover of the enzyme cuts both strands at a single site. However, some type II enzymes behave differently from this paradigm. For example, SfiI interacts with two copies of its recognition site before DNA cleavage, while FokI seems to need two copies of the protein at one site. The objective of this study is to elucidate the range of different behaviours displayed by type II restriction enzymes; to assess the prevalence of enzymes in this category that act differently from the paradigms such as EcoRV and EcoRI; and to characterise the mode of action of BspMI and some other enzymes that act in non-standard ways, with respect to their protein assemblies and their mechanisms for DNA recognition and cleavage.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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