BBSRC Portfolio Analyser
Award details
Characterisation of the inv gene and its protein product
Reference
G10386
Principal Investigator / Supervisor
Professor Judith Goodship
Co-Investigators /
Co-Supervisors
Professor Tom Strachan
Institution
Newcastle University
Department
School of Cell and Molecular Biosciences
Funding type
Research
Value (£)
147,968
Status
Completed
Type
Research Grant
Start date
12/08/1998
End date
12/08/2001
Duration
36 months
Abstract
In all vertebrates there are invariant left-right asymmetries with the cardiac apex, stomach and spleen on the left and liver on the right. The inv mouse is the only model in which these asymmetries are consistently reversed and is therefore key to understanding left-right specification. We have cloned the gene responsible for this phenotype, it has 15 tandem ankyrin like repeat units followed by novel sequence. We plan to generate antibodies to the ankyrin like and novel regions of the gene and to use these to identify the cellular localisation of the protein. We will study its temporospatial expression pattern. The presence of ankyrin like repeats indicates interaction with other proteins and these will be sought using the yeast two hybrid system. Orthologues will be identified for evolutionary comparison and functional studies.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search