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The mechanism of action of 4E-binding proteins in yeast
Reference
G10379
Principal Investigator / Supervisor
Professor John McCarthy
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
184,248
Status
Completed
Type
Research Grant
Start date
01/10/1998
End date
01/10/2001
Duration
36 months
Abstract
Yeast and mammalian cells regulate gene expression at the level of translational initiation via proteins that bind the cap-binding protein eIF4E. We have recently established a new system for studying these eIF4E-binding proteins (4E-BPs) in S. cerevisiae which can be used to examine their mechanism of action in considerable detail. We will: 1) Characterise the relationship between binding-domain structure and the eIF4E-binding affinity and specificity of the respective yeast and human 4E- BPs (p20 and 4E-BP1) using techniques of mutagenesis surface plasmon resonance and X-ray crystallography; 2) Determine how the binding of p20/4E-BP1 to eIF4E in yeast modulates translation in vivo and characterise the mechanism regulating this interaction; 3) Use the yeast-based regulatory system to screen for mammalian genes that encode kinases capable of modulating 4E-BP function.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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