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Chromosome partitioning in Pseudomonas putida
Reference
G10277
Principal Investigator / Supervisor
Professor Christopher Thomas
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
205,996
Status
Completed
Type
Research Grant
Start date
01/02/1999
End date
01/08/2002
Duration
42 months
Abstract
We will investigate the process of chromosome partitioning which depends on the ParA and ParB genes of Pseudomonas putida. We will create plasmids consisting of the chromosome replication origin and the adjacent gid operon encoding ParA and ParB so as to be able to manipulate required essential cis-acting sequences (centromere-like sequences) and determine whether they are sufficient to direct active partitioning. We will also dissect the ParA and ParB proteins by creating site directed mutations to determine what happens when these are overproduced in wildtype P. putida. The phenotypic consequences of these mutations will be assessed in lysed, fixed bacteria using fluorescent signals generated by antibodies or fluorescent oligonucleotides. This will allow us to test the hypothesis that partitioning proceeds through a pairing between chromosomes and that ParA/ParB interaction and phosphorylation is an essential step in this process.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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