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Genetic dissection of a vertebrate centromere
Reference
G10112
Principal Investigator / Supervisor
Dr William Brown
Co-Investigators /
Co-Supervisors
Institution
University of Nottingham
Department
Sch of Biology
Funding type
Research
Value (£)
163,150
Status
Completed
Type
Research Grant
Start date
01/10/1998
End date
01/10/2002
Duration
48 months
Abstract
Current ideas about eukaryotic centromeres indicate that the centromere proteins CENP-C and CENP-A play key roles in centromere function. We propose to test these ideas by manipulating the genes encoding CENP-C and CENP-A in the hyper recombinogenic chicken cell line DT40. We will engineer fusion proteins between CENP-C and the bacterial DNA binding protein lex A and use this to localise CENP- C to ectopic positions in the chicken genome. We will examine the consequences for the behaviour of the chicken chromosomes by cytogenetic and immuno-cytochemical techniques. We will engineer a conditional loss of function mutant of the CENP-A gene and then examine the behaviour of the chicken chromosomes under restrictive conditions by cytogenetic and immuno-cytochemical techniques.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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