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Positioning of the U5 snRNA with exon sequences during pre-mRNA splicing
Reference
G10086
Principal Investigator / Supervisor
Professor Raymond O'Keefe
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
154,907
Status
Completed
Type
Research Grant
Start date
01/12/1998
End date
01/12/2001
Duration
36 months
Abstract
The U5 snRNA is required for alignment of exons for the second step of pre-mRNA splicing. The mechanisms by which U5 recognises and becomes positioned with the exons at splice sites are unclear. This project will establish whether the U2 and U6 snRNAs, the proposed catalytic factors in splicing, interact with U5 to direct it to the exons. U2 and U6 mutants that block the second step of splicing will be analysed to determine if they influence exon alignment of U5 in vitro. Direct interactions between U5 and these snRNAs will be mapped at high resolution by site specific crosslinking in vitro and investigated by genetic studies in vivo. Such analysis will further our understanding of the critical cellular process of pre-mRNA splicing.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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