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Genetic and biochemical analysis of prion elimination in yeast.
Reference
G09663
Principal Investigator / Supervisor
Professor Emeritus Mick Tuite
Co-Investigators /
Co-Supervisors
Dr B Cox
Institution
University of Kent
Department
Sch of Biosciences
Funding type
Research
Value (£)
162,495
Status
Completed
Type
Research Grant
Start date
01/10/1998
End date
01/10/2001
Duration
36 months
Abstract
The realisation that PSI, a non-Mendelian genetic determinant of yeast, actually represents a prion-like state of the Sup35p protein has opened up an entirely new genetic approach to the study of prions. The [PSI] prion will be used to determine the mechanism by which certain non-mutagenic compounds (e.g. guanidine HC1) can eliminate yeast prions. The cellular response of yeast to such chemicals will be characterised paying particular attention to the stress response. The importance of heat shock proteins in the maintenance and curing of [PSI] will be defined using both in vitro and in vivo assays. Finally, genetic screens will be used to isolate non-curable forms of [PSI]. A full understanding of the mechanism of prion curing in yeast will facilitate the development of a chemical strategy for curing mammalian prions.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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