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Control of stem cell pluripotency by transcription factor Oct-4
Reference
G09504
Principal Investigator / Supervisor
Professor Austin Smith
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Stem Cell Research
Funding type
Research
Value (£)
779,498
Status
Completed
Type
Research Grant
Start date
01/04/1999
End date
01/04/2003
Duration
48 months
Abstract
The POU transcription factor Oct-4 is essential for establishment of the pluripotential stem cell lineage in the early mouse embryo. Our objectives are: (i) to investigate and define continuous requirements for Oct-4 in embryos, somatic stem cells and germ cells by temporally regulated gene deletion in vivo; (ii) to clone the key Oct-4 co-factor(s) by a cDNA complementation screen in embryonic stem (ES) cells that express Oct-4 constitutively; (iii) to identify Oct-4 target genes by a subtractive hybridisation approach using an engineered ES cell line in which expression/repression of Oct-4 is under tight inducible control.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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