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Zn(II)-homeostasis and transcriptional regulation in E. coli
Reference
G09282
Principal Investigator / Supervisor
Dr Andrew Morby
Co-Investigators /
Co-Supervisors
Institution
Cardiff University
Department
School of Biosciences
Funding type
Research
Value (£)
145,844
Status
Completed
Type
Research Grant
Start date
01/03/1998
End date
01/03/2001
Duration
36 months
Abstract
This study is centred on three genes involved in the control of Zn(II)-availability in E. coli zntA and yhhn are transcriptionally controlled by alterations in Zn(II) concentrations in their environment. zntA transcription is induced by increasing Zn(II), in contrast, yhhn is induced in the absence of Zn(II). A regulatory protein (YhdM) has been identified which binds to the zntA promoter; protein/DNA and protein/DNA/Zn(II) interactions will be studied. The transcriptional activity of yhhn and yhhm will be investigated using reporter genes and by direct PCR mediated transcript analysis. Gene(s) encoding regulatory proteins that mediate that the Zn(II)-repression of yhhn will be sought using genetically selective protein/DNA interaction screens. The preliminary assignment of YhhM and YhhN as Zn(II)-importers will be confirmed and measurements of Zn(II)- uptake made.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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