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Genetic and molecular approaches towards the isolation of the pollen determinant of self incompatiblity in petunia

ReferenceG09242
Principal Investigator / Supervisor Dr Timothy Robbins
Co-Investigators /
Co-Supervisors
Institution University of Nottingham
DepartmentSch of Biosciences
Funding typeResearch
Value (£) 119,860
StatusCompleted
TypeResearch Grant
Start date 25/05/1998
End date 25/11/2000
Duration30 months

Abstract

This proposal addresses the isolation of the pollen-specificity determinant of the gametophytic self incompatibility system (pollen-S) of the Solanaceae. A heterologous transposon tagging strategy in Petunia hybrida has identified a number of candidate mutations. A recent model suggests that mutations in pollen-S may be lethal for the male gametophyte and a modified transposon tagging scheme is proposed that addresses this possibility. This scheme takes advantage of transposons that have been shown to be linked to the S locus of Petunia hybrida. A parallel approach relies on a fine scale genetic and physical map around pollen-S using a novel scheme for the pre-selection of recombinants. This map will allow a number of pollen-S candidates to be tested and will provide the basis for a position based cloning strategy. The identification of pollen-S will provide unique insights into molecular recognition in plants and provide important opportunities for the manipulation of plant breeding behaviour.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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