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Metabolic engineering of recombinant protein sialylation in animal cells by co-expression of CMP-NeuAC synthetase and transporter genes
Reference
G09240
Principal Investigator / Supervisor
Professor David James
Co-Investigators /
Co-Supervisors
Dr Anthony Baines
Institution
University of Kent
Department
Sch of Biosciences
Funding type
Research
Value (£)
190,816
Status
Completed
Type
Research Grant
Start date
29/06/1998
End date
29/06/2001
Duration
36 months
Abstract
The pharmacokinetics and bioactivity of recombinant therapeutic glycoproteins can be directly affected by terminal sialylation of their N-glycans. This is the most variable N- glycan modification and is therefore a major source of recombinant glycoprotein heterogeneity. The objective of this programme is to overcome metabolic constraints on sialylation, recently elucidated at Kent, by metabolic engineering of animal cells with recently cloned cDNA's encoding the CMP-NeuAc synthetase and transporter. The outcomes of this research will be (i) an understanding of metabolic control of N- glycosylation in animal cells and (ii) creation of host cells for expression of highly sialylated recombinant glycoprotein products.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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