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Gene regulation mediated by post-termination ribosomes
Reference
G09227
Principal Investigator / Supervisor
Professor John McCarthy
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
164,344
Status
Completed
Type
Research Grant
Start date
02/04/1998
End date
02/04/2001
Duration
36 months
Abstract
The S. cerevisiae YAP1 and YAP2 genes encode AP1-like transcription factors involved in the cellular responses to various stresses including those caused by oxidation and heavy metals. They are subject to posttranscriptional control mediated by upstream open reading frames (uORFs) in their leaders. The most remarkable aspect of this control is that the YAP2 uORFs act as mRNA destablising elements, whose influence is transferable to other genes. This project focuses on the mechanism linking the interactions between posttermination ribosomes that have translated the YAP uORFs with the subsequent translation and turnover of the mRNA. We will: 1. characterise the properties of uORFs that determine their function in promoting reinitiation, ribosome release and accelerated mRNA decay; 2. analyse the pathway of uORF-dependent mRNA decay and determine how this is coupled to the translational termination process; 3. study the response of these uORF-coupled mechanisms to stress conditions.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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