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Molecular cloning of a gametophytic gene required for correct division asymmetry and cell fate in Arabidopsis
Reference
G08788
Principal Investigator / Supervisor
Professor David Twell
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Medicine - Pathology
Funding type
Research
Value (£)
178,013
Status
Completed
Type
Research Grant
Start date
21/09/1998
End date
21/09/2001
Duration
36 months
Abstract
The key objective of this proposal is to achieve the molecular cloning and characterization of the GEMINI80 gene which is required for correct division asymmetry and cell fate determination at pollen mitosis I (PMI). The specific aims are (1) to use map based cloning and targeted transposon-tagging to isolate the GEMINI80 gene; (2) to monitor precisely the developmental events leading to altered division symmetry in gem80, including analysis of nuclear migration, spindle orientation and cytokinesis; and (3) to use GUS and GFP marker gene technologies to monitor how altered division symmetry affects cell fate in gem80; and (4) to determine allelism between gem80 and five related gemini mutants.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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