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Ribosome fates following translation termination in yeast

Reference	G08463
Principal Investigator / Supervisor	Professor Ian Stansfield
Co-Investigators / Co-Supervisors	Professor John McCarthy
Institution	University of Aberdeen
Department	School of Medical Sciences
Funding type	Research
Value (£)	140,445
Status	Completed
Type	Research Grant
Start date	01/04/1998
End date	30/09/2001
Duration	42 months

Abstract

The mechanisms controlling the behaviour of eukaryotic ribosomes subsequent to translation termination are poorly defined, yet post-termination ribosomes play important roles in aspects of gene expression including translation initiation via ribosome recycling, translational regulation (e.g. GCN4 in Saccharomyces cerevisiae and cytomegalovirus gp48), and nonsense-mediated mRNA decay. We will use a combination of in vivo and in vitro approaches, including in vivo screens, to identify cis-acting elements and trans-acting factors which control the fate of post-termination ribosomes and thus affect these processes. The influence of the length and structure of the reading frame being translated on post-termination events will also be tested. The composition and mRNA binding behaviour of post- termination ribosomal subunits will be examined using novel biochemical techniques, including an in vitro `scanning trap' designed to freeze ribosomal movement 3' of the stop codon.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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