Award details

Is Sfi1 the prototype for many endonucleases or is it unique?

Reference	G08275
Principal Investigator / Supervisor	Professor Steve Halford
Co-Investigators / Co-Supervisors
Institution	University of Bristol
Department	Biochemistry
Funding type	Research
Value (£)	43,348
Status	Completed
Type	Research Grant
Start date	01/02/1998
End date	01/02/1999
Duration	12 months

Abstract

SfiI differs from all other restriction enzymes characterised to date. Its recognition sequence is 13 bp long while most restriction sites are 4-6 bp. It is a tetrameric protein while the other enzymes are dimers. Moreover, instead of a dimer cutting both strands at one site, SfiI has to interact with two copies of its target sequence before being able to cleave DNA. It then cuts both strands at both sites, excising the intervening DNA in a manner reminiscent of many recombinases. To see if the behaviour is unique to SfiI, or whether SfiI is the prototype for a class of endonucleases, the properties of several other restriction enzymes that have esoteric recognition sites will be analysed. These may also have to span two recognition sites before DNA cleavage but even if this is not so, they pose problems in DNA recognition that lack any precedence from conventional enzymes like EcoRV.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search