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Combinatorial mutagenesis and domain structure of the MerR regulator of Tn501
Reference
G07943
Principal Investigator / Supervisor
Professor Nigel Brown
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
186,038
Status
Completed
Type
Research Grant
Start date
01/12/1997
End date
31/07/2001
Duration
43 months
Abstract
The regulator of mercury resistance genes, MerR, is the archetype of a new family of prokaryotic transcriptional regulators with related N-terminal DNA binding regions. We wish to test the hypothesis that MerR acts through Hg-dependent interconversion of a repressor and activator form and begin the structural determination of the protein. We will use recombinational mutagenesis to generate new constitutive activator mutations, constitutive repressor mutations and to alter the metal ion specificity, and we will investigate expressing MerR subdomains for structural studies. The properties of the mutant and subdomain proteins will be characterised.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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