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Identification of mutants defective in light and / or plastid signalling to nuclear photosynthetic gene expression in Arabidopsis
Reference
G07782
Principal Investigator / Supervisor
Dr Enrique Lopez-Juez
Co-Investigators /
Co-Supervisors
Institution
Royal Holloway, Univ of London
Department
Biological Sciences
Funding type
Research
Value (£)
109,358
Status
Completed
Type
Research Grant
Start date
15/09/1997
End date
31/08/2000
Duration
35 months
Abstract
We aim to characterise a set of cue (CAB underexpressor) mutants of Arabidopsis, previously isolated by the applicant in the laboratory of J Chory (Salk Inst.), to identify one primarily affected in either light or plastid signalling regulating nuclear photogene expression. To this end, in each cue mutant, we will: a) Examine the morphology and a marker protein in etioplasts. b) Analyse photogene expression under diagnostic conditions. c) Attempt to rescue the phenotypes of cues with a gun1 mutation, which partly uncouples nuclear photogene expression from plastid functional status. d) Use a set of available CAB3 promoter fragment:luciferase reporters, to identify promoter regions through which each cue mutation acts, and compare them with regions responsible for plastid regulation. This project will define the target for a subsequent one, centred on cloning one CUE gene.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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