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Heat flux as an on-line process control variable to optimise growth and product fidelity in recombinant animal cells

Reference	G07389
Principal Investigator / Supervisor	Dr Ross King
Co-Investigators / Co-Supervisors	Dr Peter Evans
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	155,655
Status	Completed
Type	Research Grant
Start date	13/01/1997
End date	13/01/1999
Duration	24 months

Abstract

The study aims to prove that heat flux is a realistic process control variable in optimising growth of genetically engineered animal cells and fidelity of their heterologous protein production. The objectives are to (i) establish theoretically the relationship for mixed catabolism between the flux for heat and those for substrates/products; (ii) test predictions for biomass and target protein production using continuous culture; (iii) optimise production by substrate additions on demand from heat flux changes; (iv) find relation between heat flux, physiology and protein quality. The model systems are CHO cell lines secreting constitutively Interferon-gamma and inducibly cytochrome b5.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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