Award details

Increasing the amount of transgene generated translatable mRNA

Reference	G07302
Principal Investigator / Supervisor	Professor Christopher Whitelaw
Co-Investigators / Co-Supervisors
Institution	The Roslin Institute
Department	Gene Expression and Development
Funding type	Research
Value (£)	162,258
Status	Completed
Type	Research Grant
Start date	01/04/1997
End date	01/04/2000
Duration	36 months

Abstract

The objective of this proposal is to generate improved hybrid-constructs capable of targeting high-level protein production to mammary cells. This will be achieved by incorporating RNA sequence elements from hepatitis B virus S gene, herpes simplex virus thymidine kinase gene and the bovine preprolactin gene into constructs encoding either human alpha-1- antitrypsin or factor IX. Mammary specificity will be directed by ovine beta-lactoglobulin sequences. The expression potential of genomic- and cDNA-based modified constructs will be evaluated by comparison to the parental constructs. A comparison of their ability to enhance protein production by stable transfection of cultured cell lines in vitro and in transgenic mice in vivo will enable a rationale design of future expression vectors which will be broadly applicable in all aspects of gene transfer technology.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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