Award details

Role of the Brachyury protein in mesoderm formation

Reference	G07003
Principal Investigator / Supervisor	Dr David Stott
Co-Investigators / Co-Supervisors	Dr Michael Clinton, Professor Hugh Woodland
Institution	University of Warwick
Department	Biological Sciences
Funding type	Research
Value (£)	150,464
Status	Completed
Type	Research Grant
Start date	30/05/1997
End date	30/05/2000
Duration	36 months

Abstract

The Brachyury gene is known, from the phenotypes of mutants, to be essential for the normal process of mesoderm formation in vertebrates. Thus, Brachyury can be seen as a key gene, cloning of which allows experimental access to the process of gastrulation. The Brachyury product has been shown to be a nuclear protein capable of sequence-specific DNA binding and transcriptional activation, but the essential targets of Brachyury function are not known. We propose cloning mouse genes which are directly regulated by the Brachyury protein. This work will involve synthesis of a conditionally active Brachyury protein in embryonic stem cells and comparative analysis of RNA populations before and after activation of Brachyury function. Knowledge of the targets of Brachyury will allow the construction of testable models describing mesoderm formation in mammals.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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