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Structure-function relationships of a novel developmentally regulated CCAAT box transcription factor

Reference	G06890
Principal Investigator / Supervisor	Professor Matt Guille
Co-Investigators / Co-Supervisors	Professor Geoff Kneale
Institution	University of Portsmouth
Department	Sch of Biological Sciences
Funding type	Research
Value (£)	169,482
Status	Completed
Type	Research Grant
Start date	06/01/1997
End date	06/01/2000
Duration	36 months

Abstract

The CCAAT motif is found in the promoters of a quarter of eukaryotic genes and several binding activities for it are known. A CCAAT box transcription factor (CBTF) undetectable in nuclei or on chromatin until GATA-2 transcription begins. Evidence strongly suggests that CBTF is novel. We will analyse the DNA-binding, subunit interaction and transcription activation domains of CBTF. We will use deletions, point mutants and chimaeras of CBTF made in E. coli and embryos. Thus we will understand how CBTF's functions are affected by developmental regulation, the mechanism of its action and the relationship of CBTF to other CCAAT factors.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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