Award details

Genetic and biochemical analysis of mechanisms regulating RNA stability in vivo

Reference	G06882
Principal Investigator / Supervisor	Dr Kenneth McDowall
Co-Investigators / Co-Supervisors
Institution	University of Leeds
Department	Inst of Integrative & Comparative Biolog
Funding type	Research
Value (£)	170,225
Status	Completed
Type	Research Grant
Start date	15/10/1996
End date	15/10/1999
Duration	36 months

Abstract

The long-term aim of this project is to elucidate the mechanisms regulating RNA decay, a fundamental but poorly understood aspect of gene regulation. Using E. coli as a model system, I propose to use a genetic selection procedure I have developed to identify novel factors that regulate the actions of RNaseE and PNPase, two ubiquitous enzymes having central roles in mRNA decay, and 3' polyadenylation of RNA, a universal modification that is a major determinant of RNA stability. In addition, established biochemical approaches will be used to investigate further the relationship between the structure and composition of an RNaseE::PNPase complex and the coordinated decay of RNA in vivo, to identify RNA structures recognised by an essential arginine-rich RNA-binding site (ARRBS) within the RNaseE protein, and to elucidate the role of these RNA structures in determining the decay fate of RNA in vivo.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search