BBSRC Portfolio Analyser
Award details
RNA-protein and protein-protein interactions in translation termination
Reference
G06869
Principal Investigator / Supervisor
Professor Emeritus Mick Tuite
Co-Investigators /
Co-Supervisors
Institution
University of Kent
Department
University of Kent Department
Funding type
Research
Value (£)
185,859
Status
Completed
Type
Research Grant
Start date
01/04/1997
End date
31/03/2000
Duration
36 months
Abstract
The two essential subunits of the yeast translation termination factor (eRF) have been identified; eRF1 and eRF3. It is proposed that eRF1 binds to mRNA and this binding is facilitated by eRF3. In this project the eRF subunit interactions will be explored using two hybrid studies. The eRF domain(s) responsible for ribosome binding will be identified and the identity of the ribosomal binding site proteins determined. Finally, the RNA binding properties of the two subunits will be studied and the importance of mRNA primary sequence in modulating the eRF:RNA binding properties analysed by in vitro selection and amplification of random aptamers.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search