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The role of the yeast WH12 in coordinating cell growth and proliferation
Reference
G06153
Principal Investigator / Supervisor
Professor Peter Edwin Sudbery
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
168,611
Status
Completed
Type
Research Grant
Start date
01/10/1996
End date
01/10/1999
Duration
36 months
Abstract
Preliminary work is described which shows that yeast whi2 mutants fail to enter Go, while whi2p overexpression leads to filamentous growth. In whi2 mutants G1 cyclin synthesis is deregulated during starvation, accounting for the continued division observed. Two mutants, gin3 and gin6 have been isolated which are synthetically lethal with whi2 and probably act in the PCK1/MAP kinase pathway. The GIN3 and GIN6 genes will be cloned and characterised. Possible genetic interactions of WHI2 with members of the PCK1/MAP kinase pathway CLN3, BCK2, GRR1, ELM1-3 and PHD 1 will be investigated. Biochemical approaches based on epitope-tagged Whi2p will be used to examine its intracellular location, possible interaction with the G1 cyclins or the p34 kinase, DNA binding and its effects on CLB synthesis or activity. Finally, the function of a possible actin-binding motif will be assessed.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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