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A genetic analysis of translation initiation at an IRES in Schizosaccharomyces pombe
Reference
G06125
Principal Investigator / Supervisor
Dr E Mellor
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Biochemistry
Funding type
Research
Value (£)
150,940
Status
Completed
Type
Research Grant
Start date
03/06/1996
End date
03/12/1999
Duration
42 months
Abstract
We have isolated a gene containing an internal ribosome entry segment (IRES) that facilitates translation initiation 720b from the 5'end of the mRNA in the yeast Schizosaccharomyces pombe. We want to exploit the presence of an IRES in a tractable genetic system which resembles higher eukaryotes in many aspects of its molecular biology to enhance our understanding of the regulation of translation initiation in eukaryotes. We intend (i) to characterise the IRES on the Spcpf1 gene (ii) to introduce mutations into the IRES and to ask if compensatory mutations at the 3'end of the 18S rRNA will restore translation initiation and (iii) to set up genetic screens to isolate trans acting factors required for IRES function.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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