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A strategy for generating nested sets of multi-megabase chromosomal deletions in ES cells by cre/loxP recombination
Reference
G05898
Principal Investigator / Supervisor
Dr Andrew Smith
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Stem Cell Research
Funding type
Research
Value (£)
196,784
Status
Completed
Type
Research Grant
Start date
06/01/1997
End date
06/01/2000
Duration
36 months
Abstract
The proposal is for the further exploration of a recently developed strategy for generating large scale chromosomal rearrangements in embryonic stem cells based on a combination of gene targeting and Cre/loxP site-specific recombination. The aim will be to generate a series of multimegabase deletions between loxP sites targeted to mapped locations on one or several chromosomes. These deletions will be selected in cultured ES cells after transient expression of the Cre recombinase. Successful germ line transmission using these ES clones will allow the derivation of mice partially haploid for defined chromosomal regions of their genome. These should provide a potentially important resource for functional analyses of the genome.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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