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Zinc metabolism in Escherichia coli: molecular analysis of zin a novel zinc inducible operon
Reference
G05844
Principal Investigator / Supervisor
Dr Andrew Morby
Co-Investigators /
Co-Supervisors
Institution
Cardiff University
Department
School of Biosciences
Funding type
Research
Value (£)
92,819
Status
Completed
Type
Research Grant
Start date
01/10/1996
End date
15/01/1999
Duration
27 months
Abstract
This study will characterise a novel zinc responsive operon (zinA) situated on the E. coli chromosome. The response of zinA to Zn, and a range of other metal ions, will be studied using transcriptional reporter genes and by directly visualising protein/DNA complexes involving the operator/promoter region. The metal binding characteristics of ZinA N-terminal (150 amino acids) will be determined and its metal ion specificity modified using site-directed mutagenesis and techniques of forced evolution. zinA will be functionally inactivated by directed recombinational insertion of a resistance marker into the gene. zin strains of E. coli will be analysed phenotypically for Zn-tolerance, sensitivity and alterations in Zn-accumulation.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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