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Identification of DNase-1 hypersensitive sites correlated with preprotachykinin-A gene expression
Reference
G04956
Principal Investigator / Supervisor
Professor John Quinn
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Veterinary Pathology
Funding type
Research
Value (£)
97,305
Status
Completed
Type
Research Grant
Start date
01/01/1996
End date
01/02/1998
Duration
25 months
Abstract
Study of preprotachykinin-A gene expression was hampered by the lack of a clonal cell line which expressed the endogenous gene. The publication of the RINm5F cell line supplied such a cell line. We will perform DNAase 1 hypersensitive mapping of the rat preprotachykinin-A (rPPT) gene in both rPPT expressing and non expressing RIN cells. This analysis will be used to determine key regulatory regions of the promoter for further study and the construction of a transgene which demonstrates physiological promoter activity. Understanding the transcriptional regulation of this gene might suggest strategies for intervention in conditions exhibiting hyperalgesia and neurogenic inflammation as well as regulation in the developing pancreas.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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