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Regulation of respiratory metabolism in the free-living nitrogen-fixing bacterium Azotobacter vinelandii
Reference
G04903
Principal Investigator / Supervisor
Professor Robert K Poole
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
197,741
Status
Completed
Type
Research Grant
Start date
01/03/1996
End date
01/03/1999
Duration
36 months
Abstract
Azotobacter vinelandii is a free-living, aerobic, nitrogen-fixing bacterium. The oxidase activity of cytochrome bd provides `respiratory protection' for nitrogenase from damage by oxygen. Using molecular genetic methods, this collaborative research (with NFL) will: (1) determine whether A. vinelandii has the genes to encode one or two terminal oxidases (CYO and/or CCO) in addition to the cytochrome bd, and determine their function by mutant construction; (2) study the regulation of expression of oxidase genes; (3) investigate how CYDR, deduced to an FNR/CRP-like transcription factor, regulates expression of oxidase genes and other genes involved in nitrogen fixation.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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