BBSRC Portfolio Analyser
Award details
Chromosomal mapping of proteins HMG14/17 and HMG1/2
Reference
G04511
Principal Investigator / Supervisor
Professor Colyn Crane-Robinson
Co-Investigators /
Co-Supervisors
Institution
University of Portsmouth
Department
Sch of Biological Sciences
Funding type
Research
Value (£)
170,019
Status
Completed
Type
Research Grant
Start date
25/10/1995
End date
07/04/1999
Duration
41 months
Abstract
There is conflicting evidence regarding the chromosomal distribution of HMG14/17 and HMG1/2. We aim to resolve these discrepancies using experience gained in mapping acetylated core histones. The methodology is to crosslink intact cells with formaldehyde (to avoid disturbance of native structures), isolate DNA/protein adducts on CsCl gradients, immunoprecipitate chromatin fragments using affinity-purified polyclonal antibodies, reverse the crosslinks and then probe the DNA with unique genomic sequences from the gene/locus under study.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search