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Novel strategies for identifying and analysing genes controlling cell proliferation and differentiation in Arabidopsis
Reference
G04197
Principal Investigator / Supervisor
Professor James Murray
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Institute of Biotechnology
Funding type
Research
Value (£)
193,457
Status
Completed
Type
Research Grant
Start date
06/04/1995
End date
06/04/1998
Duration
36 months
Abstract
Few methods are currently available for obtaining mutations in essential plant cellular processes. We propose two new strategies for screening and analysing mutations affecting cell proliferation and differentiation in Arabidopsis. The first uses FLP-mediated mitotic recombination to generate genetic mosaics with clones of marked homozygous mutant cells in a background of heterozygous, wild-type cells. This is appropriate both for analysing the role of known embryonic lethal genes on later cell behaviour, and for screening for new mutations affecting such cellular processes. The second uses transposition followed by FLP- mediated deletions to generate nested chromosomal deficiencies from a fixed point in each direction along a chromosome.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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