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Function of the amino terminal region I of sigma-N in enhancer-dependent transcription
Reference
G04069
Principal Investigator / Supervisor
Professor Martin Buck
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
151,333
Status
Completed
Type
Research Grant
Start date
01/08/1995
End date
30/11/1998
Duration
40 months
Abstract
The RNA polymerase holoenzyme containing Sigma-N responds to remotely bound activator proteins. The NH2-50aa Region I of Sigma-N is needed for this and is required in order that the holoenzyme locally unwinds the DNA. How Region I in concert with core RNA polymerase controls the activity of the DNA binding domain present in the C-terminus of Sigma-N will be investigated. Region I will be purified, its activity in trans examined, and its accessibility in holoenzyme assessed. DNA contacts which respond to Region I will be precisely mapped in Sigma-N. Whether Region I simply prepares the promoter for activation or contributes a contact surface for the activator should be distinguished.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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