Award details

Monitoring control of glycosylation microheterogeneity in recombinant proteins expressed in animal cells

Reference	G03681
Principal Investigator / Supervisor	Professor Nigel Jenkins
Co-Investigators / Co-Supervisors	Dr Anthony Baines, Professor Alan Bull
Institution	University of Kent
Department	University of Kent Department
Funding type	Research
Value (£)	245,233
Status	Completed
Type	Research Grant
Start date	01/04/1994
End date	01/05/1997
Duration	37 months

Abstract

In this proposal we will investigate the complex problems of glycan microheterogeneity, i.e. what controls the type and ratios of individual sugars within each glycan structure? The main questions to be addressed are: 1. To what extent does the microheterogeneity of the common N-glycan structures on secreted and membrane recombinant glycoproteins change during culture? 2. Are there common oligosaccharide motifs that are more susceptible to change? 3. How do these glycan differences relate to specific steps along the glycosylation biosynthetic pathway? 4. What physiological changes can be made to cells in order to ensure minimal glycan heterogeneity throughout a batch or fed-batch culture, or to select for specific glycan structures?

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search