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Investigation of two novel trans-acting factors from Avena fatua aleurone cells which bind an alpha-amylase promoter
Reference
G02401
Principal Investigator / Supervisor
Dr Colin Lazarus
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Biological Sciences
Funding type
Research
Value (£)
190,454
Status
Completed
Type
Research Grant
Start date
01/02/1995
End date
31/12/1998
Duration
47 months
Abstract
Clones encoding two trans-acting factors that bind specifically to a functionally important cis- element in the promoter of gibberellin-regulated alpha-amylase genes have been isolated from an Avena fatua aleurone cDNA expression library. We aim to : 1. determine the DNA-binding specificities of each protein using DNase 1 footprinting and gel retardation of sequences with wild-type and in vitro mutated proteins; 2. determine their roles in alpha-amylase transcription by suppression of their activities in aleurone protoplasts using antibodies or by expression of antisense or dominant negative mutants; 3. identify proteins with which they interact by methods of interaction cloning.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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