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Use of site-specific recombination to circumvent position effect in transgenic animals

Reference	G02343
Principal Investigator / Supervisor	Dr J Bishop
Co-Investigators / Co-Supervisors	Dr Deborah Fowlis, Professor Austin Smith
Institution	University of Edinburgh
Department	Inst of Cell, Animal and Population Biol
Funding type	Research
Value (£)	244,916
Status	Completed
Type	Research Grant
Start date	01/07/1995
End date	05/12/1999
Duration	52 months

Abstract

Most genes introduced randomly into the mammalian genome exhibit variable expression dependent on the site of integration. This increases the cost of experiments up to 10-fold and is particularly significant in the case of farm animals. The problem may be avoided by site-specific recombination, mediated by an integrase such as bacteriophage P1 Cre recombinase, into a site (loxP) introduced at a chromosomal location that permits high-level expression. The rate of DNA integration by this route has been too low to be of use. We suggest that this is so because the rate of excision is high, and propose a procedure that may give a high rate of integration. We will test this proposal (1) in ES cells and (2) in microinjected one-cell embryos.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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